ArrayVision Fast Serum Biomarker Chip Tutorial

 

Data Analysis of SBC slide with ArrayVision FAST

 

  1. Open protocol from the Protocol Editor/File/Select Protocol/SBC
  2. Load the Dy647 (biotin) scan into the first channel and the Dy547 (FITC) scan into the second channel using the () button. Once images are loaded click “Register images”.
  3. To place template on spots click on Alignment. Next place curser onto top left spotfrom the top left array and left click. This should now place display the template over the array. Click OK on the Protocol Editor.
  4. Sample the data.
  5. Export data from ArrayVision FAST using the “Export to MS Excel” function. This is located as a button () on the toolbar. Once activated continue by clicking “Export”.
  6. Data will now be in MS Excel. Save and close file to desired location before moving on to SBC Analysis Workbook Macro.

ArrayVision FAST SBC Macro—Installing the Macro

 

  1. Open Excel file: “SBC data analysis for ArrayVision”. This will place a button on the toolbar for SBC analysis. Once button has been installed do not move this Excel file. If moved, an error upon clicking the button will indicate “file could not be found”.
  2. One can reestablish this link by first deleting the old SBC button. To delete button, open Excel/right click on the SBC button/choose customize. Select the Tool bars Tab. Delete SBC Analysis Workbook. Close Excel and now open the “SBC data analysis for ArrayVision” Excel file from its new location.
  3. To change the appearance of this button—place curser over button and right click. Select “Customize”. Right click once again over the button and select “Text Only (Always)”.
  4. From now on this button will appear on the Excel toolbar as “SBC Analysis Workbook”.

Using the SBC Analysis Workbook

 

  1. To activate macro from Excel, left click once on the toolbar button. Security within Excel must be at medium (recommended) or low. If security is high a window like the one below will appear—



  2. To run the macro first click on the Analyze button. Once this operation has finished click on the Graph button.
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© 2005 Whatman Schleicher & Schuell BioScience

 

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