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The S&S Two-Color
Labeling and Fluorescent Detection Kit is designed to label two protein
samples. The labeled proteins are pooled and probed against arrayed
antibodies in a competitive binding assay, and detected using indirect
fluorescence.
Two-Color Labeling and Detection Kit
How It Works
The first pad on the slide is probed with a mixture of two different protein samples, each labeled with a different hapten; the second pad is probed with the same two protein samples but with the haptens reversed.
The normalized intensity for each element of each pad is calculated as the average of the biotin- and fluorescein-labeled derived intensities from a two-pad experiment.
The ratio between the signal intensity at each spot corresponds to the concentration ratio of the proteins found in the two samples.
This method is attractive for antibody chips as it takes into account any hapten-specific differences in antigen-antibody interactions.
Contents of Two-Color Labeling & Detection Kit
The kit contains the Universal Linkage System™ (ULS™) chemistry to label samples containing approximately 250 µg of protein in serum, plasma, or a whole cell lysate. The kit is designed to label two different protein samples, each with a different hapten. Sufficient labeling reagent is provided to perform a hapten swapping experiment.
Contents List: Biotin-ULS™ 20 µl Fluorescein-ULS™ 20 µl 10x Protein Labeling Buffer 80 µl 10x KREA stop 80 µl Streptavidin-DY™647 Conjugate 150 µl Anti-Fluorescein Antibody-DY™547 conjugate 350 µl Micro Bio-Spin® Chromatography Columns 8 User Manual
Two-Color Labeling & Detection Kit - Ordering Information
To order contact Customer Service at: 1-800-245-4024 or 603-352-3810
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Benefits of the
two-color labeling assay:
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