Serum Protein Profiles of Cancer Patients

 

 

Serum proteins from cancer patients and corresponding age- and gender-matched controls were labeled with Biotin-ULS and Fluorescein-ULS, respectively, then pooled and probed against the Serum Biomarker Chip.

The competitive binding was detected using both streptavidin-Dy647 and anti-fluorescein antibody-Dy547 conjugates. Spot-finding, background corrections, and two-channel data sampling was done using ArrayVision FAST software. 		  The SBC Analysis Workbook is a Microsoft® Excel file that converts fluorescent data into numerical values that represent the abundance of antigen in Sample A relative to Sample B. The average spot intensities for each analyte was plotted to reveal the over- and underabundant serum biomarkers in the cancer patient (y-axis) compared to serum of a healthy individual (x-axis) for bladder, breast, colon, and prostate cancer.

Click on the links below to see graphs.
Bladder Cancer
  
Colon Cancer
Breast Cancer
  
   
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© 2005 Whatman Schleicher & Schuell

 

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